human b lymphoblastoid cell line 721 221  (ATCC)

Journal: Molecules

Article Title: A Marine Natural Product, Harzianopyridone, as an Anti-ZIKV Agent by Targeting RNA-Dependent RNA Polymerase

doi: 10.3390/molecules29050978

Figure Lengend Snippet: Efficacy of HAR against ZIKV in 3 different cell lines.

Article Snippet: Human glioblastoma cell line SNB19 (CRL-2219, ATCC, Manassas, VA, USA) was obtained from ATCC.

Techniques:

Journal: Molecules

Article Title: A Marine Natural Product, Harzianopyridone, as an Anti-ZIKV Agent by Targeting RNA-Dependent RNA Polymerase

doi: 10.3390/molecules29050978

Figure Lengend Snippet: The antiviral effects of HAR in SNB19, Vero, and A549 cells. ( A ) The ZIKV titers in SNB19, Vero, and A549 cells were quantified by real-time PCR (MOI = 0.2). The supernatant was obtained from cells treated with HAR at the indicated concentrations at 48 h post-infection (hpi). ( B ) Representative images of the plaque-forming assay. On Vero cells, the plaque-forming assay was used to confirm the ZIKV titers in the supernatant. The supernatant was obtained from SNB19, Vero, and A549 cells treated with HAR at the indicated concentrations at 48 h post-infection (hpi).

Article Snippet: Human glioblastoma cell line SNB19 (CRL-2219, ATCC, Manassas, VA, USA) was obtained from ATCC.

Techniques: Real-time Polymerase Chain Reaction, Infection

Journal: Molecules

Article Title: A Marine Natural Product, Harzianopyridone, as an Anti-ZIKV Agent by Targeting RNA-Dependent RNA Polymerase

doi: 10.3390/molecules29050978

Figure Lengend Snippet: HAR inhibits the expression of ZIKV proteins. Western blotting analysis of protein expression of ZIKV NS5 and E in the cell lysates of SNB19, Vero, and A549 for the anti-ZIKV activity of HAR or DMSO at the indicated concentrations at 48 h post-infection (hpi) (MOI = 0.2).

Article Snippet: Human glioblastoma cell line SNB19 (CRL-2219, ATCC, Manassas, VA, USA) was obtained from ATCC.

Techniques: Expressing, Western Blot, Activity Assay, Infection